APA
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Clementino, M., Cavalcante, K. F., Viana, V. A. F., de Oliveira Silva, D., Damasceno, C. R., de Souza, J. F., … Havt, A. (2022). Detection of SARS‐CoV‐2 in different human biofluids using the loop‐mediated isothermal amplification assay: A prospective diagnostic study in Fortaleza, Brazil. MedRxiv.
Chicago/Turabian
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Clementino, M., Karene Ferreira Cavalcante, V. A. F. Viana, Dayara de Oliveira Silva, Caroline Rebouças Damasceno, Jessica Fernandes de Souza, R. D. Gondim, et al. “Detection of SARS‐CoV‐2 in Different Human Biofluids Using the Loop‐Mediated Isothermal Amplification Assay: A Prospective Diagnostic Study in Fortaleza, Brazil.” medRxiv (2022).
MLA
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Clementino, M., et al. “Detection of SARS‐CoV‐2 in Different Human Biofluids Using the Loop‐Mediated Isothermal Amplification Assay: A Prospective Diagnostic Study in Fortaleza, Brazil.” MedRxiv, 2022.
BibTeX Click to copy
@article{m2022a,
title = {Detection of SARS‐CoV‐2 in different human biofluids using the loop‐mediated isothermal amplification assay: A prospective diagnostic study in Fortaleza, Brazil},
year = {2022},
journal = {medRxiv},
author = {Clementino, M. and Cavalcante, Karene Ferreira and Viana, V. A. F. and de Oliveira Silva, Dayara and Damasceno, Caroline Rebouças and de Souza, Jessica Fernandes and Gondim, R. D. and Jorge, D. M. and Magalhães, L. M. and Arruda, E. and da Justa Pires Neto, Roberto and Medeiros, M. and Santos, A. and Magalhães, P. and Mello, L. P. and Arruda, E. and Lima, A. and Havt, A.}
}
We adopted the reverse‐transcriptase‐loop‐mediated isothermal amplification (RT‐LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS‐Cov‐2) in patient samples. Two primer sets for genes N and Orf1ab were designed to detect SARS‐CoV‐2, and one primer set was designed to detect the human gene Actin. We collected prospective 138 nasopharyngeal swabs, 70 oropharyngeal swabs, 69 salivae, and 68 mouth saline wash samples from patients suspected to have severe acute respiratory syndrome (SARS) caused by SARS‐CoV‐2 to test the RT‐LAMP in comparison with the gold standard technique reverse‐transcription quantitative polymerase chain reaction (RT‐qPCR). The accuracy of diagnosis using both primers, N5 and Orf9, was evaluated. Sensitivity and specificity for diagnosis were 96% (95% confidence interval [CI]: 87–99) and 85% (95% CI: 76–91) in 138 samples, respectively. Accurate diagnosis results were obtained only in nasopharyngeal swabs processed via extraction kit. Accurate and rapid diagnosis could aid coronavirus disease 2019 (COVID‐19) pandemic management by identifying, isolating, and treating patients rapidly.
