APA
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Prata, M., Cavalcante, P., da Silva, A. D., de Medeiros, P. H. D., de Assis Júnior, E. M., Reyes, M. A. V., … Lima, A. (2019). Alanyl-glutamine Protects Against Damage Induced by Enteroaggregative Escherichia coli Strains in Intestinal Cells. Journal of Pediatric Gastroenterology and Nutrition.
Chicago/Turabian
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Prata, M., P. Cavalcante, A. D. da Silva, P. H. D. de Medeiros, Eudmar M de Assis Júnior, M.A.V. Reyes, J. Quetz, et al. “Alanyl-Glutamine Protects Against Damage Induced by Enteroaggregative Escherichia Coli Strains in Intestinal Cells.” Journal of pediatric gastroenterology and nutrition (2019).
MLA
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Prata, M., et al. “Alanyl-Glutamine Protects Against Damage Induced by Enteroaggregative Escherichia Coli Strains in Intestinal Cells.” Journal of Pediatric Gastroenterology and Nutrition, 2019.
BibTeX Click to copy
@article{m2019a,
title = {Alanyl-glutamine Protects Against Damage Induced by Enteroaggregative Escherichia coli Strains in Intestinal Cells},
year = {2019},
journal = {Journal of pediatric gastroenterology and nutrition},
author = {Prata, M. and Cavalcante, P. and da Silva, A. D. and de Medeiros, P. H. D. and de Assis Júnior, Eudmar M and Reyes, M.A.V. and Quetz, J. and Clementino, M. and Ribeiro, S. A. and Lima, Ila Fernanda N. and Lima-Júnior, R. and Havt, A. and Guerrant, R. and Lima, A.}
}
Background: Enteroaggregative Escherichia coli (EAEC) is an important pathogen causing enteric infections worldwide. This pathotype is linked to malnutrition in children from developing countries. Alanyl-glutamine (Ala-Gln) is an immune modulator nutrient that acts during intestinal damage and/or inflammation. This study investigated the effect of EAEC infection and Ala-Gln on cell viability, cell death, and inflammation of intestinal epithelium cells (IEC-6). Methods: Cells were infected with an EAEC prototype 042 strain, an EAEC wild-type strain isolated from a Brazilian malnourished child, and a commensal E coli HS. Gene transcription and protein levels of caspases-3, -8, and -9 and cytokine-induced neutrophil chemoattractant 1 (CINC-1/CXCL1) were evaluated using RT-qPCR, western blot analysis, and ELISA. Results: Infections with both EAEC strains decreased cell viability and induced apoptosis and necrosis after 24 hours. Ala-Gln supplementation increased cell proliferation and reduced cell death in infected cells. Likewise, EAEC strain 042 significantly increased the transcript levels of caspases-3, -8, and -9 when compared to the control group, and Ala-Gln treatment reversed this effect. Furthermore, EAEC induced CXCL1 protein levels, which were also reduced by Ala-Gln supplementation. Conclusion: These findings suggest that EAEC infection promotes apoptosis, necrosis, and intestinal inflammation with involvement of caspases. Supplementation of Ala-Gln inhibits cell death, increases cell proliferation, attenuates mediators associated with cell death, and inflammatory pathways in infected cells.
